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To: Jack Home From: Sent Mon 9/10/2012 7:21:15 PM Subject: Re: Dinochicken meeting proposal Thank you Jack! I will pass this along to Jeffrey. Take care, Lesley On Sep 10, 2012, at 3:00 PM, Jack Homer wrote: Hi Jeffrey, I've conducted the Doodle Poll and everyone is interested in getting together sooner rather than later, so we arc looking at October 24-25. I'm wondering if you would be able to help with funding this "Dinochicken Summit" and what you would need from me. Also would very much like for you to be here if its at all possible, as you could then see what all is known and what is not. All the best, Jack On Sep 1, 2012, at 11:13 AM wrote: You as well Sent from my iPhone On Sep 1, 2012, at 12:53 PM, Jack Homer a> wrote: Thanks much appreciated! Have a great weekend! Jack On Sep 1, 2012, at 10:38 AM, Lesley Groff wrote: Thank you!! Passing this on to Jeffrey. Sent from my iPhone On Sep 1, 2012, at 12:18 PM, Jack Horner wrote: EFTA_R1_00832038 EFTA02159900 Dear Jeffrey, First off, many thanks for the invitation to your ranch, I had a great time, especially spending time with you and the girls, and seeing your Cretaceous sediments and the old railroad. I don't get many chances to spend time with non paleontologists or see places and things like that these days. I talked to my post does and Co- PI when I returned and got the scoop on the transgenic chickens situation, for as you know I was very surprised to hear that such a thing existed since my co-researchers were saying they didn't. As it turns out, although there are such a thing, there is simply no established protocol to create them, and they, at present do not pass on their characteristics, so in effect are useless for our project. What it is that we need to do is to be able to target a specific region of DNA, and that technology is not yet available in birds, but is expected within a year or two, so within our time frame. We hope someone else will do it first so we can use it, as this technology (homologous recombination technology, aka knockout technology) is essential to the success of this project. We do not aim to generate chickens that express an extraneous gene (a transgene), but we plan on finding the very small changes in the regulation of existing genes EFTA_R1_00832039 EFTA02159901 that curb tail, hand, and tooth development. In order to make these very small changes in the chicken genome, homologous recombination technology needs to be developed. Anyway, this is the sort of stuff we will discuss in our summit meeting, and I very much appreciate your considering the funding of this meeting. It is obviously the most important first step, and I thank you for suggesting we do it. Please give all the girls my very best wishes, and to you, whom I envy, Thanks again, Jack 9.1.12 Proposal Attached and Separate (attachment) <THE DINOCHICKEN PROJECT `SUMMIT' MEETING PROPOSAL.docx> THE DINOCHICKEN PROJECT `SUMMIT' MEETING PROPOSAL The overall goals of the `Dinochicken Project' are to `reverse engineer' an avian dinosaur (in this case, a chicken or quail) to EFTA_R1_00832040 EFTA02159902 have morphological characteristics of a non-avian dinosaur. In its purest form, the project entails reactivating dormant or latent genetic pathways that would generate atavistic characters. The characters to be modified initially are those identified previously as key differences between avian and non-avian dinosaurs: presence of teeth, hands/claws instead of wings, and a long true tail. A key component to any project is identifying what is already known and establishing connections with other scientists who may have relevant information already in hand. There are 6 PIs who will make the research decisions, and another 11 people who we know have expertise, and are interested in participating. The current, unpublished knowledge of these other researchers would definitely help to decide the research directions of the various PI lead teams. I am proposing to bring 12 people to Montana for the two day `summit' and we have another 5 that live here, so the meeting will include 17 research scientists plus a couple of MSU EFTA_R1_00832041 EFTA02159903 graduate students who would help with logistics. The summit meeting would be held at the Museum of the Rockies, Montana State University. Participants arc currently working on Doodle.com to determine a meeting time prior to the end of 2012. The link is attached in the event that you could attend: http://doodle.com/ed2xvmvx8 u3t9fxs Summit Meeting Proposed Participants Montana State University Jack Homer Lead PI (Paleontologist) Christa Merzdorf Co PI (Developmental geneticist) (tail development) Anne Blackwell Post-Doc (Developmental geneticist) (tail development) Dana Rashid Post-Doc (Developmental geneticist) (tail regeneration) Dan Post-Doc (Developmental geneticist) EFTA_R1_00832042 EFTA02159904 'wo Graduate Student Assistants &n EFTA_R1_00832043 EFTA02159905